The characteristic patterns of these highly abundant proteins are used to reliably and accurately identify a particular microorganism by matching the respective pattern with an extensive open database to determine the identity of the microorganism down to the species level.
The MALDI Biotyper System:
Highly Accurate
Applicable to a Wide Range of Microorganisms
Much Faster than Traditional Methods
Cost Effective
Robust and Easy to Use
Searching for the culprit?
Fast and reliable strain-specific identification of microorganisms, like bacteria, yeasts, or fungi, is mandatory in clinical routine monitoring, food processing quality control or environmental and taxonomical research. Benefit from ultra-fast analysis time and a simple consistent analysis workflow with high differentiation power.
The molecular fingerprint
Discover bacteria’s identity by its own unique molecular composition. In a MALDI-TOF mass spectrometer, proteins and peptides are arranged in a spectrum with increasing mass – revealing a characteristic peak pattern, even for reliable differentiation of strains: The individual fingerprint.
Sample amount is not critical
The sample material can be a single colony from overnight culture or a centrifuged portion of a liquid culture. Biological material is prepared as smear or extract respectively, representing millions of cells roughly, although a minimum of 10 cells can be measured accurately on a standard MALDI sample target plate.
Thông số kỹ thuật
Very sensitive, amounts of roughly 105
cells are required
No initial assessment, each sample handled in the same way:
No gram staining, oxidase- or any other tests and conditions choice
Samples
Bacteria (gram+ / gram-), yeasts, fungi, spores
Fields of application
Clinical routine monitoring, environmental and taxonomical research, food processing quality control
Features identification, taxonomical classification, or dereplication of microorganisms
Sample preparation / handling
Very simple and rapid preparation.
Instrumentation
Fast measurements with high reproducibility on a microflexTM, autoflexTM or ultraflexTM MALDI-TOF mass spectrometer
Software
MALDI Biotyper, COMPASSTM for FLEX series incl. flexAnalysisTM
Speed of analysis
Minutes from colony to result for a single sample, approx. 1.5 hours from colony to result for 96 samples
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